Environmental
DNA

 

Environmental DNA (eDNA) is genetic material shed by living organisms into the soil, air, or water. Using advanced technologies, samples of this genetic material can be analyzed to tell us which species could be present in an area. Since 2020, the River Institute’s FINS project, in collaboration with the Lougheed Lab at Queen’s University, has incorporated the collection of eDNA into the project’s surveys, offering a unique opportunity to compare eDNA-based monitoring with traditional sampling methods using seine nets. In 2022, the River Institute acquired funding to purchase a real-time qPCR instrument to begin building in-house eDNA capacity. In 2024, the River Institute initiated planning for the construction of a new eDNA laboratory as part of the establishment of the Climate Change Observatory of Ontario (ECCO-Ontario), a new research partnership that includes Queen’s University, the River Institute, and the Mohawk Council of Akwesasne, among other institutions.

Contact

Matt Windle
mwindle[at]riverinstitute.ca

Objectives

  • Utilize eDNA to improve our understanding of species distributions
  • Support the development and refinement of eDNA methods and sampling protocols
  • Provide training and build capacity

Partners & Collaborators

Queen’s University, Mohawk Council of Akwesasne

Funders

Previous & Current

  • Trottier Family Foundation
  • Canada Foundation for Innovation
  • Fisheries and Oceans Canada
  • Ontario Power Generation
  • Private donors

Publications

PEER-REVIEWED ARTICLE

Enhancing metabarcoding of freshwater biotic communities: A new online tool for primer selection and exploring data from 14 primer pairs

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PEER-REVIEWED ARTICLE

Nearshore fish diversity changes with sampling method and human disturbance: comparing eDNA metabarcoding and seine netting along the Upper St. Lawrence River

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